منابع مشابه
Preparation and properties of spinach ribonuclease.
Many investigators (l-6) have reported that crude extracts from a large number of plant sources are capable of catalyzing the depolymerization of ribonucleic acid. Only those plant ribonucleases obtained from pea leaves (7, S), tobacco leaves (9), and ryegrass (10) have been extensively purified, and none has been prepared as an entirely pure protein. It was a major objective of the work descri...
متن کاملA Practical Preparation of Ribonuclease-S and the Action of Subtilisin on Ribonuclease-B ‡
All preparations of ribonuclease-S so far reported have employed limited digestion of ribonuclease-A with subtilisin,' a bacterial proteinase from B. subtilis described by Giintelberg and Ottesen.' The original supply of this latter enzyme is now exhausted. Another sample of a crystalline proteinase from B. subtilis (NOVO Enzyme) has been shown to differ from the original in several respects,' ...
متن کاملPreparation and properties of two active forms of ribonuclease dimer.
When bovine pancreatic ribonuclease A is lyophilized from 507, acetic acid and the product is dissolved in phosphate buffer, enzymically active dimers, tetramers, and higher aggregates may be isolated by gel filtration on Sephadex G-75 (1). The molecular weights of the fractions isolated from the Sephadex column were established by ultracentrifugation. In 0.2 M phosphate buffer the monomer is t...
متن کاملA Method for the Preparation of "protease-free" Crystalline Ribonuclease
A method is described for the preparation of crystalline ribonuclease free from all measurable traces of proteolytic enzymes.
متن کاملOn the preparation of bovine pancreatic ribonuclease A.
Many studies on ribonuclease would benefit if reproducible, purified samples of the enzyme were readily available. The present report summarizes experiments on the preparation and storage of chromatographically purified bovine pancreatic ribonuclease A.’ Much of the earlier work on ribonuclease was done with a single, large, commercial batch of material (Armour Lot 381059) which was isolated fr...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0264-6021
DOI: 10.1042/bj1730005